Desulfococcus multivorans Medium

[所属分类:培养基配方] [发布时间:2020-6-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfococcus multivorans Medium

培养基配方
Composition per liter:
NaCl......................................................................10.0g
MgSO4 ·7H2O...............................................................2.0g
CaSO4 .....................................................................1.0g
NH4Cl .....................................................................1.0g
Yeast extract..............................................................1.0g
K2HPO4 ....................................................................0.5g
Sodium lactate (70% solution).............................................3.5mL
Preparation of Medium: Add components, except FeSO4 ·7H2O,
ascorbic acid, and thioglycollic acid, to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Sparge with 80% N2  + 20% CO2
for 10–15 min. Add FeSO4 ·7H2O, ascorbic acid, and thioglycollic acid.
Mix thoroughly. Continue to sparge with 80% N2  + 20% CO2 and ad-
just pH to 7.4. Anaerobically distribute into tubes or flasks. Autoclave
for 10 min at 10 psi pressure–115°C.
Use: For the cultivation of Desulfococcus multivorans.