Desulfobulbus Medium

[所属分类:培养基配方] [发布时间:2020-6-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfobulbus Medium

培养基配方
Composition per liter:
Sodium propionate.............................................................1.85g
Na2SO4 ........................................................................1.5g
(NH4)2SO4 ....................................................................1.24g
NaCl...........................................................................0.6g
L -Cysteine·HCl·H2O..........................................................0.5g
KH2PO4 ........................................................................0.3g
MgSO4 ·7H2O..................................................................0.12g
CaCl2 ·2H2O .................................................................0.08g
Trace minerals...............................................................10.0mL
Vitamin solution.............................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Trace Minerals:
Composition per liter:
Nitrilotriacetic acid........................................................12.8g
FeSO4 ·7H2O..................................................................0.3g
CoCl2 ·2H2O..................................................................0.1g
MnCl2 ·4H2O .................................................................0.1g
ZnCl2 ........................................................................0.1g
CuCl2 .......................................................................0.02g
H3BO3 .......................................................................0.01g
Na2MoO4 .....................................................................0.01g
NiSO4 ·6H2O.................................................................2.6mg
Na2SeO3 .....................................................................1.7mg
Preparation of Trace Minerals: Add nitrilotriacetic acid to
500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5
with KOH. Add remaining components. Add distilled/deionized water
to 1.0L.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl...............................................................0.01g
Thiamine·HCl ................................................................5.0mg
Riboflavin ...................................................................5.0mg
Nicotinic acid................................................................5.0mg
Calcium pantothenate .........................................................5.0mg
p-Aminobenzoic acid...........................................................5.0mg
Thioctic acid ................................................................5.0mg
Biotin .......................................................................2.0mg
Folic acid....................................................................2.0mg
Cyanocobalamin ...............................................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation  of  Medium: Add  components,  except  L -
cysteine·HCl·H2O, to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Gently heat and bring to boiling. Cool to 25°C
under 85% N2 + 15% CO2 . Add  L -cysteine·HCl·H2O. Mix thoroughly.
Adjust pH to 7.2 with KHCO3 . Anaerobically distribute into tubes or
flasks under 85% N2 + 15% CO2 . Autoclave for 15 min at 15 psi pres-
sure–121°C. Adjust pH to 7.0, if necessary.
Use:  For the cultivation and maintenance of Desulfobulbus elongatus.