Desulfobacterium cetonicum Medium

[所属分类:培养基配方] [发布时间:2020-6-4] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfobacterium cetonicum Medium

培养基配方
Composition per 1011.0mL:
Solution A..................................................................950.0mL
Solution C...................................................................10.0mL
Solution D....................................................................40.0mL
Solution E ...................................................................10.0mL
Solution B (Trace elements solution SL-10) ....................................1.0mL
pH 7.2–7.4 at 25°C
Solution A:
Composition per 950.0mL:
NaCl............................................................................10.0g
Na2SO4 ..........................................................................2.8g
KH2PO4 ..........................................................................0.7g
MgCl2 ·6H2O.....................................................................0.3g
NH4Cl ...........................................................................0.3g
CaCl2 ·2H2O....................................................................0.05g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 950.0mL. Mix thoroughly. Sparge with 80% N2
+ 20% CO2 . Autoclave for 15 min at 15 psi pressure–121°C.
Solution B (Trace Elements Solution SL-10):
Composition per liter:
FeCl2 ·4H2O.....................................................................1.5g
CoCl2 ·6H2O .................................................................190.0mg
MnCl2 ·4H2O..................................................................100.0mg
ZnCl2 .........................................................................70.0mg
Na2MoO4 ·2H2O ................................................................36.0mg
NiCl2 ·6H2O...................................................................24.0mg
H3BO3 .........................................................................6.0mg
CuCl2 ·2H2O ..................................................................2.0mg
HCl (25% solution)............................................................10.0mL
Preparation of Solution B (Trace Elements Solution SL-10):
Add FeCl2 ·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add dis-
tilled/deionized water and bring volume to 1.0L. Add remaining com-
ponents. Mix thoroughly. Sparge with 100% N2 . Autoclave for 15 min
at 15 psi pressure–121°C.
Solution C:
Composition per 10.0mL:
Sodium butyrate................................................................1.2g
Preparation of Solution C:  Add sodium butyrate to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Sparge
with 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Solution D:
Composition per 40.0mL:
NaHCO3 .........................................................................2.0g
Preparation of Solution D:  Add NaHCO3 to distilled/deionized
water and bring volume to 40.0mL. Mix thoroughly. Sparge with 100%
N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Solution E:
Composition per 10.0mL:
Na2S·9H2O.......................................................................0.3g
Preparation of Solution E:  Add Na2S·9H2O to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100%
N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium:  Prepare and dispense medium under 80%
N2 + 20% CO2 . Aseptically and anaerobically combine 950.0mL of
sterile solution A with 1.0mL of sterile solution B, 10.0mL of sterile
solution C, 40.0mL of sterile solution D, and 10.0mL of sterile solution
E. Mix thoroughly. Check that final pH is 7.2–7.4.
Use: For the cultivation of Desulfobacterium cetonicum.