Desulfitobacterium dehalogenans Medium

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfitobacterium dehalogenans Medium

培养基配方
Composition per liter:
Solution A...............................................................955.0mL
Solution B................................................................25.0mL
Solution C................................................................20.0mL
Solution A:
Composition per 955.0mL:
Na2HPO4 ...................................................................2.2g
Yeast extract..............................................................2.0g
3-Chloro-4-hydroxyphenylacetic acid.......................................1.5g
L -Cysteine·HCl·H2O......................................................0.7g
NH4Cl .....................................................................0.5g
KH2PO4 ...................................................................0.44g
MgCl2 ·6H2O ..............................................................0.2g
CaCl2 ...................................................................25.0mg
Wolfe's mineral solution ................................................10.0mL
Wolfe's Mineral Solution:
MgSO4 ·7H2O...............................................................3.0g
Nitrilotriacetic acid......................................................1.5g
NaCl.......................................................................1.0g
MnSO4 ·H2O................................................................0.5g
FeSO4 ·7H2O ..............................................................0.1g
CoCl2 ·6H2O...............................................................0.1g
CaCl2 .....................................................................0.1g
ZnSO4 ·7H2O...............................................................0.1g
CuSO4 ·5H2O..............................................................0.01g
A1K(SO4)2 ·12H2O ........................................................0.01g
H3BO3 ....................................................................0.01g
Na2MoO4 ·2H2O............................................................0.01g
Preparation of Wolfe's Mineral Solution:  Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components one at a time. Add distilled/deion-
ized water to 1.0L.
Preparation of Solution A:  Add components, except  L -cyste-
ine·HCl·H2O, to distilled/deionized water and bring volume to
955.0mL. Mix thoroughly. Gently heat and bring to boiling. Cool to
room temperature while sparging with 90% N2 + 10% CO2 . Adjust pH
to 7.3. Add  L -cysteine·HCl·H2O. Autoclave for 15 min at 15 psi pres-
sure–121°C.
Solution B:
Composition per 25.0mL:
Sodium pyruvate...........................................................2.2g
Preparation of Solution B: Add sodium pyruvate to distilled/de-
ionized water and bring volume to 25.0mL. Mix thoroughly. Filter ster-
ilize. Sparge with 100% N2 .
Solution C:
Composition per 20.0mL:
NaHCO3 ...................................................................1.0g
Preparation of Solution C: Add NaHCO3  to distilled/deionized
water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.
Sparge with 100% CO2 .
Preparation of Medium: Aseptically and anaerobically combine
955.0mL of sterile solution A with 25.0mL of sterile solution B and
20.0mL of sterile solution C. Mix thoroughly. Aseptically and anaero-
bically distribute into sterile tubes or flasks.
Use: For the cultivation of Desulfitobacterium dehalogenans.