Desulfacinum Medium (DSMZ Medium 1100)

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfacinum Medium
(DSMZ Medium 1100)

培养基配方
Composition per liter:
NaCl .........................................................................7.0g
MgCl2 ·6H2O .................................................................3.1g
Na2SO3 .......................................................................3.0g
KCl...........................................................................0.5g
Yeast extract.................................................................0.5g
NH4Cl ........................................................................0.3g
KH2PO4 .......................................................................0.2g
CaCl2 ·2H2O .................................................................0.1g
Resazurin ...................................................................0.5mg
Na-lactate solution.........................................................10.0mL
NaHCO3  solution............................................................10.0mL
Na2S·9H2O solution.........................................................10.0mL
Trace elements solution SL-10 with EDTA......................................1.0mL
Selenite/tungstate solution .................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Na-lactate Solution :
Composition per 10.0mL:
Na-lactate....................................................................2.0g
Preparation of Na-lactate Solution:  Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Cool to room temperature.
NaHCO3 Solution :
Composition per 10.0mL:
NaHCO3 .......................................................................1.5g
Preparation of NaHCO3 Solution:  Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge
with 20% CO2 + 80% H2 . Autoclave for 15 min at 15 psi pressure–
121°C. Cool to room temperature.
Na2S·9H2O Solution :
Composition per 10.0mL:
Na2S·9H2O.....................................................................0.5g
Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Adjust to pH 7.0. Autoclave under 100% N2 for 15 min at 15 psi pres-
sure–121°C. Cool to room temperature.
Selenite/Tungstate Solution:
Composition per liter:
NaOH...........................................................................0.5g
Na2WO4 ·2H2O.................................................................4.0mg
Na2SeO3 ·5H2O................................................................3.0mg
Preparation of Selenite/Tungstate Solution:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution SL-10 with EDTA:
Composition per liter:
FeCl2 ·4H2O..................................................................1.5g
Na2 -EDTA.....................................................................0.5g
CoCl2 ·6H2O ..............................................................190.0mg
MnCl2 ·4H2O...............................................................100.0mg
ZnCl2 ......................................................................70.0mg
Na2MoO4 ·2H2O .............................................................36.0mg
NiCl2 ·6H2O................................................................24.0mg
H3BO3 .......................................................................6.0mg
CuCl2 ·2H2O ................................................................2.0mg
HCl (25% solution)..........................................................10.0mL
Preparation of Trace Elements Solution SL-10 with EDTA:
Add FeCl2 ·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add dis-
tilled/deionized water and bring volume to 1.0L. Add remaining com-
ponents. Mix thoroughly. Sparge with 100% N2 . Autoclave for 15 min
at 15 psi pressure–121°C. Adjust pH to 7.0.
Preparation of Medium: Add components, except bicarbonate,
lactate, and sulfite solution, to distilled/deionized water and bring vol-
ume to 970.0mL. Mix thoroughly. Gently heat and bring to boiling.
Boil for 1 min. Cool while sparging with 100% N2 . Add the solid bi-
carbonate. Dispense  under  100% N2  into culture vessels. Autoclave
for 15 min at 15 psi pressure–121°C. Aseptically and anoxically add
bicarbonate, lactate, and sulfide solutions. Adjust final pH of the medi-
um to pH 7.2.
Use: For the cultivation of Desulfacinum spp.