Deoxycholate Agar

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Deoxycholate Agar

培养基配方
Composition per liter:
Agar ..............................................................................15.0g
Peptic digest of animal tissue.....................................................10.0g
Lactose............................................................................10.0g
NaCl................................................................................5.0g
K2HPO4 .............................................................................2.0g
Ferric citrate......................................................................1.0g
Sodium citrate......................................................................1.0g
Sodium deoxycholate.................................................................1.0g
Neutral Red........................................................................0.03g
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Do not autoclave. Cool to 45°–50°C. Pour into sterile Petri
dishes.
Use:  For the selective isolation, cultivation, enumeration, and differen-
tiation of Gram-negative enteric microorganisms from a variety of clin-
ical and nonclinical specimens. Escherichia coli appears as large, flat,
rose-red colonies. Enterobacter and Klebsiella species appear as large,
mucoid, pale colonies with a pink center. Proteus and Salmonella spe-
cies appear as large, colorless to tan colonies. Shigella species appear as
colorless to pink colonies. Pseudomonas species appear as irregular col-
orless to brown colonies.