Deoxycholate Agar

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Deoxycholate Agar

培养基配方
Composition per liter:
Agar..............................................................................16.0g
Lactose...........................................................................10.0g
NaCl...............................................................................5.0g
Pancreatic digest of casein........................................................5.0g
Peptic digest of animal tissue ....................................................5.0g
K2HPO4 ............................................................................2.0g
Ferric citrate.....................................................................1.0g
Sodium citrate.....................................................................1.0g
Sodium deoxycholate................................................................1.0g
Neutral Red......................................................................0.033g
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Do not autoclave. Cool to 45°–50°C. Pour into sterile Petri
dishes.
Use:  For the selective isolation, cultivation, enumeration, and differentia-
tion of Gram-negative enteric microorganisms from a variety of clinical
and nonclinical specimens. Escherichia coli appears as large, flat, rose-red
colonies. Enterobacter and Klebsiella species appear as large, mucoid,
pale colonies with a pink center. Proteus and Salmonella species appear as
large, colorless to tan colonies. Shigella species appear as colorless to pink
colonies. Pseudomonas species appear as irregular colorless to brown col-
onies.