Demi-Fraser Broth

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Demi-Fraser Broth

培养基配方
Composition per liter:
NaCl...........................................................................20.0g
Tryptose.......................................................................10.0g
Na2HPO4 ........................................................................9.6g
Beef extract....................................................................5.0g
Yeast extract...................................................................5.0g
LiCl............................................................................3.0g
KH2PO4 ........................................................................1.35g
Esculin ........................................................................1.0g
Acriflavin·HCl...............................................................12.5mg
Nalidixic acid................................................................10.0mg
Ferric ammonium citrate supplement............................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder and supple-
ment from BD Diagnostic Systems.
Ferric Ammonium Citrate Supplement:
Composition per 10.0mL:
Ferric ammonium citrate.........................................................0.5g
Preparation of Ferric Ammonium Citrate Supplement:  Add
ferric ammonium citrate to distilled/deionized water and bring volume
to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except ferric ammoni-
um citrate supplement, to distilled/deionized water and bring volume
to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 10.0mL of sterile ferric ammonium citrate sup-
plement. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation of Listeria species from food and environmen-
tal samples.