Defined Medium for Rhodopseudomonas

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Defined Medium for Rhodopseudomonas

培养基配方
Composition per liter:
Malic acid.....................................................................4.0g
(NH4)2SO4 .....................................................................1.0g
K2HPO4 ........................................................................0.9g
KH2PO4 ........................................................................0.6g
MgSO4 ·7H2O...................................................................0.2g
CaCl2 ·2H2O ................................................................0.075g
EDTA..........................................................................0.02g
FeSO4 ·7H2O.................................................................0.012g
Thiamine......................................................................1.0mg
Biotin .....................................................................0.015mg
Trace elements................................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Trace Elements:
Composition per 250.0mL:
H3BO3 ..........................................................................0.7g
MnSO4 ·H2O.....................................................................0.4g
Na2MoO4 ·2H2O ................................................................0.19g
ZnSO4 ·7H2O...................................................................0.06g
CoCl2 ·6H2O ..................................................................0.05g
Cu(NO3)2 ·3H2O ...............................................................0.01g
Preparation of Trace Elements: Add components to distilled/deion-
ized water and bring volume to 250.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use:  For the cultivation and maintenance of Rhodobacter capsulatus.