Defined Glucose Medium EMSY-1

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Defined Glucose Medium EMSY-1
Composition per liter:
Na2HPO4 .......................................................................1.79g
KH2PO4 .........................................................................1.7g
Citric acid.....................................................................0.5g
NH4Cl .........................................................................0.43g
MgSO4 ·7H2O...................................................................0.41g
CaCl2 ·2H2O...................................................................0.04g
NaCl...........................................................................0.03g
FeCl3 ·6H2O..................................................................4.84mg
Glucose solution ............................................................100.0mL
Yeast extract solution........................................................10.0mL
TK6-3 solution.................................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
Glucose .......................................................................10.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract..................................................................0.4g
Preparation of Yeast Extract Solution:  Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
TK6-3 Solution:
Composition per liter:
ZnSO4 ·7H2O..................................................................1.45g
CuSO4 ·5H2O..................................................................0.76g
MnSO4 ·H2O...................................................................0.31g
H3BO3 ........................................................................0.19g
Na2MoO4 ·2H2O ...............................................................0.17g
KI............................................................................0.04g
H2SO4 (1N solution) ..........................................................1.0mL
Preparation of TK6-3 Solution: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except glucose solu-
tion and yeast extract solution, to distilled/deionized water and bring
volume to 890.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool rapidly to 25°C.
Aseptically add 100.0mL of sterile glucose solution and 10.0mL of
sterile yeast extract solution. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation and maintenance of Xanthomonas campestris.