Decarboxylase Basal Medium (BAM M44)

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Decarboxylase Basal Medium
(BAM M44)

培养基配方
Composition per liter:
Peptone or gelysate..........................................................5.0g
Yeast extract................................................................3.0g
Glucose......................................................................1.0g
Bromcresol Purple...........................................................0.02g
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Adjust pH so that it will be
6.5 ± 0.2 after sterilization. Distribute into 16 × 150mm screw-capped
tubes in 5.0mL volumes. Autoclave medium with loosely capped tubes for
10 min at 15 psi pressure–121°C. Screw the caps on tightly for storage and
after inoculation.
Use : For the cultivation and differentiation of bacteria based on their
ability to decarboxylate the amino acid. As the basal medium for argi-
nine broth, lysine broth, and ornithine broth. Bacteria that decarboxy-
late arginine, lysine, or ornithine turn the medium turbid purple. The
unsupplemented decarboylase basal medium is used as a control.