D/E-Neutralizing Broth (Dey/Engley-Neutralizing Broth)

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

D/E-Neutralizing Broth
(Dey/Engley-Neutralizing Broth)

培养基配方
Composition per liter:
Glucose.....................................................................10.0g
Soybean lecithin.............................................................7.0g
Na2S2O3 ·5H2O...............................................................6.0g
Tween™ 80....................................................................5.0g
Pancreatic digest of casein..................................................5.0g
NaHSO3 ......................................................................2.5g
Yeast extract................................................................2.5g
Sodium thioglycolate.........................................................1.0g
Bromcresol Purple...........................................................0.02g
pH 7.6± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
in 9.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the neutralization and testing of antiseptics and disinfectants.