DCMYBA

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

DCMYBA

培养基配方
Composition per liter:
Agar ...........................................................................20.0g
Cornmeal........................................................................15.0g
Supplement solution ..........................................................100.0mL
Supplement Solution:
Composition per 100.0mL:
Glucose ........................................................................20.0g
Yeast extract....................................................................1.0g
Biotin ......................................................................100.0μg
Preparation of Supplement Solution: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add cornmeal to distilled/deionized wa-
ter and bring volume to 900.0mL. Mix thoroughly. Gently heat and
bring to boiling. Maintain at 100°C for 30 min. Filter through What-
man filter paper. Add agar to filtrate and bring volume to 1.0L with dis-
tilled/deionized water. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50–
55°C. Aseptically add 100.0mL of sterile supplement solution. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Sordaria brevicollis.