DA Medium

[所属分类:培养基配方] [发布时间:2020-6-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

DA Medium

培养基配方
Composition per liter:
NaCl..........................................................................116.9g
Agar...........................................................................15.0g
Tris-HCl......................................................................6.024g
NaHCO3 ........................................................................1.68g
MgSO4 ·7H2O..................................................................1.232g
KNO3 .........................................................................0.505g
CaCl2 ........................................................................0.033g
KH2PO4 .......................................................................0.014g
H3BO3 .........................................................................6.0mg
MnCl2 ·4H2O ................................................................99.0μg
ZnCl2 .......................................................................14.0μg
CoCl2 ·6H2O.................................................................4.76μg
CuCl2 ·2H2O .................................................................34.0ng
FeCl3 solution ...............................................................50.0mL
pH 7.5 ± 0.2 at 25°C
FeCl 3 Solution:
Composition per 50.0mL:
EDTA...........................................................................5.84mg
FeCl3 .........................................................................0.32mg
Preparation of FeCl3 Solution:  Add components to distilled/de-
ionized water and bring volume to 50.0mL. Mix thoroughly.
Preparation of Medium:  Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Distribute into screw-capped tubes. Autoclave for 15 min at 15 psi
pressure–121°C. Allow to cool in a slanted position.
Use: For the cultivation of Dunaliella bardawil.