​Czapek Yeast Extract Agar

[所属分类:培养基配方] [发布时间:2020-6-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Czapek Yeast Extract Agar

培养基配方
Composition per liter:
Sucrose......................................................................30.0g
Agar ........................................................................15.0g
Yeast extract.................................................................5.0g
K2HPO4 .......................................................................1.0g
Czapek concentrate...........................................................10.0mL
Czapek Concentrate:
Composition per liter:
NaNO3 ........................................................................30.0g
KCl............................................................................5.0g
MgSO4 ·7H2O...................................................................5.0g
FeSO4 ·7H2O...................................................................0.1g
ZnSO4 ·7H2O...................................................................0.1g
CuSO4 ·5H2O..................................................................0.05g
Preparation of Czapek Concentrate: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Aspergillus niger.