Czapek Peptone Agar

[所属分类:培养基配方] [发布时间:2020-6-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Czapek Peptone Agar

培养基配方
Composition per liter:
Sucrose...................................................................30.0g
Agar .....................................................................15.0g
Peptone....................................................................5.0g
KNO3 ......................................................................2.0g
Yeast extract..............................................................2.0g
K2HPO4 ....................................................................1.0g
KCl........................................................................0.5g
MgSO4 ·7H2O...............................................................0.5g
FeSO4 ·7H2O..............................................................0.01g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Actinoplanes campanulatus, Actinoplanes dig-
itatis, Actinoplanes italicus, Actinoplanes lobatus, Actinoplanes regularis,
Actinoplanes utahensis, Ampullariella campanulata, Micromonospora
brunnea, Micromonospora purpurea, Micromonospora purpureochro-
mogenes, Micromonospora species, Microtetraspora glauca, Micromono-
spora chalcea, Nocardia brevicatena, Pilimelia anulata, Pilimelia
terevasa, Planomonospora parontospora, Promicromonospora citrea,
Saccharomonospora caesia, Saccharomonospora viridis, Spirillospora
albida, Sporichthya polymorpha, Streptomyces yerevanensis, Thermoac-
tinomyces thalpophilus, Thermoactinomyces vulgaris, and Thermomono-
spora chromogena.