Czapek Agar with 20% Sucrose (CZA200)

[所属分类:培养基配方] [发布时间:2020-6-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Czapek Agar with 20% Sucrose
(CZA200)

培养基配方
Composition per liter:
Sucrose...........................................................................200.0g
Agar...............................................................................15.0g
NaNO3 ..............................................................................3.0g
K2HPO4 .............................................................................1.0g
KCl.................................................................................0.5g
MgSO4 ·7H20........................................................................0.5g
FeSO4 ·7H2O.......................................................................0.01g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except sucrose, to dis-
tilled/deionized water and bring volume to 700.0mL. Mix thoroughly.
In a separate flask, add sucrose to distilled/deionized water and bring
volume to 300.0mL. Mix thoroughly. Autoclave both solutions sepa-
rately for 15 min at 15 psi pressure–121°C. Cool to 45–50°C. Combine
the sterile solutions. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use:  For the cultivation of Aspergillus repens (Eurotium repens).