Czapek Agar with Peptone (ATCC Medium 522)

[所属分类:培养基配方] [发布时间:2020-6-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Czapek Agar with Peptone
(ATCC Medium 522)

培养基配方
Composition per liter:
Sucrose..........................................................................30.0g
Agar.............................................................................15.0g
Peptone ..........................................................................5.0g
NaNO3 ............................................................................3.0g
K2HPO4 ...........................................................................1.0g
KCl...............................................................................0.5g
MgSO4 ·7H2O......................................................................0.5g
FeSO4 ·7H2O.....................................................................0.01g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except sucrose, to dis-
tilled/deionized water and bring volume to 900.0mL. Mix thoroughly.
Distribute into tubes or flasks. In a separate flask, add sucrose to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave both solutions separately for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Combine the sterile solutions. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Streptomyces species. For
the cultivation of Actinoplanaceae.