Cystine Tryptone Agar, HiVeg with Carbohydrate

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cystine Tryptone Agar, HiVeg with Carbohydrate

培养基配方
Composition per liter:
Plant hydrolysate ........................................................20.0g
NaCl.......................................................................5.0g
Agar ......................................................................2.5g
L -Cystine.................................................................0.5g
Na2SO3 ....................................................................0.5g
Phenol Red...............................................................17.0mg
Carbohydrate solution....................................................50.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium, without carbohydrate solution, is available as
a premixed powder from HiMedia.
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate..............................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Adonitol, ara-
binose, cellobiose, glucose, dulcitol, fructose, galactose, inositol, lac-
tose, maltose, mannitol, raffinose, rhamnose, salicin, sorbitol, sucrose,
trehalose, xylose, or other carbohydrates may be used. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Aseptically add 50.0mL of sterile carbohydrate solution. Mix thor-
oughly. Pour into sterile Petri dishes or leave in tubes.
Use: For fermentation studies of various bacteria.