Cylindrocladium Isolation Medium

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cylindrocladium Isolation Medium

培养基配方
Composition per liter:
Agar ..........................................................................20.0g
Glucose .......................................................................15.0g
KH2PO4 .........................................................................1.0g
KNO3 ...........................................................................0.5g
MgSO4 ·7H2O....................................................................0.5g
Yeast extract...................................................................0.5g
Chloramphenicol solution......................................................10.0mL
Chlortetracycline solution....................................................10.0mL
Thiabendazole solution........................................................10.0mL
Tergitol NPX ® (Union Carbide).................................................1.0mL
Chloramphenicol Solution:
Composition per 10.0mL:
Chloramphenicol.................................................................0.1g
Ethanol (95% solution)........................................................10.0mL
Preparation of Chloramphenicol Solution: Add chlorampheni-
col to 10.0mL of ethanol. Mix thoroughly. Filter sterilize.
Chlortetracycline Solution:
Composition per 10.0mL:
Chlortetracycline...............................................................0.04g
Ethanol, absolute...............................................................5.0mL
Preparation of Chlortetracycline Solution: Add chlortetracy-
cline to 5.0mL of ethanol. Mix thoroughly. Bring volume to 10.0mL
with distilled/deionized water. Filter sterilize.
Thiabendazole Solution:
Composition per 10.0mL:
Thiabendazole...................................................................1.0mg
Preparation of Thiabendazole Solution: Add thiabendazole to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Filter sterilize tergitol NPX. Add compo-
nents—except tergitol NPX, thiabendazole solution, chloramphenicol
solution, and chlortetracycline solution—to distilled/deionized water
and bring volume to 969.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile tergitol NPX, thiabendazole solu-
tion, chloramphenicol solution, and chlortetracycline solution. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Cylindrocladium species.