CYE Agar, Buffered (Charcoal Yeast Extract Agar, Buffered)

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CYE Agar, Buffered
(Charcoal Yeast Extract Agar, Buffered)

培养基配方
Composition per liter:
Agar...................................................................................17.0g
ACES buffer (N-2-acetamido-2-aminoethane sulfonic acid)................................10.0g
Yeast extract..........................................................................10.0g
Charcoal, activated, acid-washed .......................................................2.0g
L- Cysteine·HCl·H2O solution.........................................................10.0mL
Fe4(P2O7)3  solution...................................................................10.0mL
pH 6.9 ± .05 at 50°C
L - Cysteine·HCl·H2O Solution:
Composition per 10.0mL:
L- Cysteine·HCl·H2O ..................................................................0.4g
Preparation of  L - Cysteine·HCl·H2O Solution: Add  L- cyste-
ine·HCl·H2O to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize.
Fe4(P2O7)3 Solution:
Composition per liter:
Fe4(P2O7)3 ..........................................................................0.25g
Preparation of Fe4 (P2O7)3 Solution: Add soluble Fe4(P2O7)3 to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize. The soluble Fe4(P2O7)3 must be kept dry and in the
dark. Do not use if brown or yellow. Prepare solutions freshly. Do not
heat over 60°C to dissolve. The mixture dissolves readily in a 50°C wa-
ter bath.
Preparation of Medium: Add components, except  L- cyste-
ine·HCl·H2O solution and Fe4(P2O7)3  solution, to distilled/deionized wa-
ter and bring volume to 980.0mL. Mix thoroughly. Gently heat to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Add 10.0mL of sterile  L- cysteine·HCl·H2O solution and 10.0mL of ster-
ile Fe4(P2O7)3  solution. Adjust pH to 6.9 at 50°C by adding 4.0–4.5mL
of 1.0 N KOH. This is a critical step. Mix thoroughly. Pour in 20.0mL
volumes into sterile Petri dishes. Swirl medium while pouring to keep
charcoal in suspension.
Use:  For the cultivation and maintenance of Legionella species and
Xylella fastidiosa.