CVA Medium (Cefoperazone Vancomycin Amphotericin Medium)

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CVA Medium
(Cefoperazone Vancomycin Amphotericin Medium)

培养基配方
Composition per liter:
Agar ............................................................................15.0g
Casein peptone...................................................................10.0g
Meat peptone.....................................................................10.0g
NaCl..............................................................................5.0g
Yeast autolysate..................................................................2.0g
Glucose ..........................................................................1.0g
NaHSO3 ...........................................................................0.1g
Sheep blood, defibrinated.......................................................50.0mL
CVA antibiotic solution.........................................................10.0mL
pH 7.0 ± 0.2 at 25°C
CVA Antibiotic Solution:
Composition per 10.0mL:
Cefoperazone....................................................................20.0mg
Vancomycin .....................................................................10.0mg
Amphotericin B...................................................................2.0mg
Preparation of CVA Antibiotic Solution: Add components to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except CVA antibiotic
solution and sheep blood, to distilled/deionized water and bring vol-
ume to 940.0mL. Mix thoroughly. Gently heat until boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile CVA antibiotic solution and sterile, defibrinated sheep
blood. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the isolation and cultivation of Campylobacter species from
clinical specimens.