Crystal Violet Pectate Medium (CVP Medium)

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Crystal Violet Pectate Medium
(CVP Medium)

培养基配方
Composition per liter:
Sodium polypectate.........................................................9.0g
Agar ......................................................................2.0g
NaNO3 .....................................................................1.0g
NaOH (1N solution)........................................................4.5mL
CaCl2 ·H2O (10% solution)................................................3.0mL
Crystal Violet (0.075% solution)..........................................1.0mL
Sodium lauryl sulfate (10% solution)......................................0.5mL
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: In a preheated blender, add 500.0mL of
boiling distilled/deionized water and the components, except sodium
polypectate and sodium lauryl sulfate solution. Blend at high speed for
15 sec. Continue blending at low speed and slowly add 9.0g of sodium
polypectate. Pour the incomplete medium into a 2L flask and add
0.5mL of sodium lauryl sulfate solution. Mix thoroughly. Cap flask
with an aluminum foil seal rather than cotton. Autoclave for 25 min at
15 psi pressure–121°C. Pour medium quickly into sterile Petri dishes.
Allow plates to dry at 25°C for 48 hr before use.
Use: For the cultivation of pectinolytic microorganisms, such as
Erwinia species, from foods.