Crystal Violet Lactose Broth

[所属分类:培养基配方] [发布时间:2020-5-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Crystal Violet Lactose Broth

培养基配方
Composition per liter:
Lactose.......................................................................5.0g
Peptic digest of animal tissue................................................5.0g
K 2 HPO 4 ....................................................................5.0g
KH 2 PO 4 ....................................................................1.0g
Crystal Violet..............................................................1.43mg
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the differentiation of pure cultures of pathogenic and non-
pathogenic staphylococci.