Crithidia Medium

[所属分类:培养基配方] [发布时间:2020-5-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Crithidia Medium

培养基配方
Composition per liter:
Sucrose.....................................................................15.0g
Pancreatic digest of casein..................................................6.0g
Yeast extract................................................................1.0g
Liver concentrate ...........................................................0.1g
Hemin solution...............................................................5.0mL
pH 7.8 ± 0.2 at 25°C
Hemin Solution:
Composition per 2.5mL:
Hemin........................................................................25.0mg
Triethanolamine (TEA)........................................................2.5mL
Preparation of Hemin Solution: Add hemin to 2.5mL trietha-
nolamine. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.8.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Slight hemin precipitate may occur.
Use: For the cultivation of Crithidia acanthocephali, Crithidia deanei,
Crithidia fasciculata, Crithidia harmosa, Crithidia hutneri, Crithidia
luciliae, Crithidia mellificae, Crithidia oncopelti, Crithidia species,
Herpetomonas samuelpessoai, Leptomonas pyrrhocoris, and Phyto-
monas davidi.