Craig’s Medium

[所属分类:培养基配方] [发布时间:2020-5-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Craig’s Medium

培养基配方
Composition per liter:
Casein acid hydrolysate................................................30.0g
Yeast extract...........................................................4.0g
K2HPO4 .................................................................0.5g
Glucose solution .....................................................20.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Glucose Solution:
Composition per 100.0mL:
Glucose.......................................................................................20.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 980.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Aseptically add glucose solution. Mix thoroughly. Aseptically
distribute into sterile tubes.
Use: For the enrichment and cultivation of Vibrio cholerae during test-
ing of enterotoxigenicity.