CPC Agar Base with Cellobiose, Colistin, and Polymyxin B

[所属分类:培养基配方] [发布时间:2020-5-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CPC Agar Base
with Cellobiose, Colistin, and Polymyxin B

培养基配方
Composition per liter:
NaCl..........................................................................20.0g
Agar..........................................................................15.0g
Cellobiose....................................................................15.0g
Peptic digest of animal tissue ...............................................10.0g
Beef extract...................................................................5.0g
Bromthymol Blue...............................................................0.04g
Cresol Red ...................................................................0.04g
Cellobiose colistin polymyxin B solution....................................100.0mL
pH 7.6 ± 0.2 at 25°C
Source: This medium, without cellobiose colistin polymyxin B solu-
tion, is available as a premixed powder from HiMedia.
Cellobiose Colistin Polymyxin B Solution:
Composition per 100.0mL:
Cellobiose....................................................................15.0g
Colistin.................................................................1,360,000U
Polymyxin B................................................................100,000U
Preparation of Cellobiose Colistin Polymyxin B Solution:
Add components to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except cellobiose co-
listin polymyxin B solution, to tap water and bring volume to 1.0L.
Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Aseptically add 100.0mL of sterile cellobio-
se colistin polymyxin B solution to 900.0 mL of the cooled agar base.
Mix thoroughly. Pour into sterile Petri dishes. Use within 7 days.
Use: For the cultivation and identification of Vibrio species from
foods.