CP Medium

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CP Medium

培养基配方
Composition per liter:
Peptone.......................................................................2.5g
Starch .......................................................................2.0g
NaNO3 .......................................................................0.38g
Tris(hydroxymethyl)aminomethane buffer.......................................0.25g
K2HPO4 .....................................................................0.038g
MgSO4 ·7H2O................................................................0.038g
CaCl2 ·2H2O................................................................0.017g
NaCl........................................................................0.013g
TC vitamins minimal eagle, 100X..............................................5.0mL
Solution 1...................................................................1.0mL
Solution 2...................................................................1.0mL
Solution 3...................................................................1.0mL
Solution 4...................................................................1.0mL
Vitamin B 12 solution........................................................0.2mL
pH 8.7 ± 0.2 at 25°C
TC Vitamins Minimal Eagle 100X:
Composition per liter:
Inositol.....................................................................2.0mg
Choline chloride.............................................................1.0mg
Folic acid...................................................................1.0mg
Nicotinamide.................................................................1.0mg
Calcium pantothenate ........................................................1.0mg
Pyridoxal....................................................................1.0mg
Thiamine·HCl ...............................................................1.0mg
Riboflavin ..................................................................0.1mg
Preparation of TC Vitamins Minimal Eagle, 100X: Add com-
ponents to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Filter sterilize.
Solution 1:
Composition per 100.0mL:
EDTA.........................................................................5.0g
KOH..........................................................................3.1g
Preparation of Solution 1: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Solution 2:
Composition per liter:
FeSO4 ·7H2O................................................................4.98g
Preparation of Solution 2: Add FeSO4 ·7H2O to distilled/deionized
water acidified with 1.0mL of H2SO4 . Bring volume to 1.0L. Mix thor-
oughly.
Solution 3:
Composition per 100.0mL:
H3BO3 ......................................................................1.14g
Preparation of Solution 3: Add H3BO3 to distilled/deionized water
and bring volume to 100.0mL. Mix thoroughly.
Solution 4:
Composition per 100.0mL:
ZnSO4 ·7H2O...................................................................0.88g
MnCl2 ·4H2O .................................................................0.144g
MoO3 .........................................................................0.071g
CoNO3 ·6H2O..................................................................0.049g
CuSO4 ·5H2O..................................................................0.016g
Preparation of Solution 4: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Vitamin B 12 Solution
Composition per 10.0mL:
Vitamin B 12 .................................................................10.0mg
Preparation of Vitamin B 12 Solution: Add vitamin B 12 to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except for vitamin so-
lutions, to distilled/deionized water and bring volume to 995.0mL. Mix
thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Aseptically add vitamin solutions. Mix thorough-
ly.
Use:  For the cultivation of Lysobacter species.