Costein’s LDS Test Medium

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Costein’s LDS Test Medium

培养基配方
Composition per liter:
Meat peptone .........................................................4.5g
Papaic digest of soybean meal.........................................2.0g
Yeast extract.........................................................3.0g
NaCl..................................................................5.0g
D -Glucose............................................................1.0g
L -Lysine monohydrochloride..........................................10.0g
Na2S2O3 ..............................................................0.2g
Fe(NH4)2 (SO4)2 ·6H2O................................................0.2g
Bromocresol Purple...................................................0.032
Agar..................................................................6.0g
pH 5.6 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes.
Overlay with viscous parrafin. Autoclave for 15 min at 15 psi pressure–
121°C. Allow tubes to solidify in a vertical position.
Use: For the identification of members of Enterobacteriaceae on the
basis of lysine decarboxylase and hydrogen sulfide production.