Cornmeal and V8 Juice Agar (ATCC Medium 309)

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cornmeal and V8 Juice Agar
(ATCC Medium 309)
Composition per liter:
Agar...........................................................................7.5g
CaCO3 .........................................................................3.0g
Cornmeal extract............................................................800.0mL
V8 juice....................................................................200.0mL
pH 5.6–6.0 at 25°C
Cornmeal Extract:
Composition per 800.0mL:
Yellow cornmeal..............................................................50.0g
Preparation of Cornmeal Extract: Add 50.0g of yellow cornmeal to
800 ml of water. Leave in1 hone hour. Filter out cornmeal through
cheesecloth. Bring volume back to 800.0mL.
Preparation of Medium: Combine components. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use:  For the cultivation and maintenance of fungi.