Cornmeal Agar, Quarter-strength (ATCC Medium 2221)

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cornmeal Agar, Quarter-strength
(ATCC Medium 2221)

培养基配方
Composition per liter:
Agar ......................................................................15.0g
Cornmeal infusion........................................................250.0mL
pH 5.6–6.0 at 25°C
Cornmeal Infusion:
Composition per liter:
Yellow cornmeal............................................................50.0g
Preparation of Cornmeal Infusion: Add cornmeal to distilled/deion-
ized water and bring volume to 1.0L. Gently heat and bring to boiling.
Simmer for 10 minutes. Filter through cheesecloth. Return volume to
1.0 liter.
Preparation of Medium: Add agar to 250.0mL cornmeal infusion
and bring volume to 1.0L with distilled/deionized water. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of fungi.