Corn Steep Liquor Medium

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Corn Steep Liquor Medium

培养基配方
Composition per liter:
Glucose.....................................................................60.0g
Corn steep liquor...........................................................40.0g
Urea.........................................................................8.0g
KH2PO4 ......................................................................5.0g
Fumaric acid ................................................................1.0g
MgSO4 ·7H2O.................................................................0.5g
Hutner’s mineral base.....................................................20.0mL
pH 7.0 ± 0.2 at 25°C
Hutner’s Mineral Base:
Composition per liter:
MgSO4 ·7H2O................................................................29.7g
Nitrilotriacetic acid.......................................................10.0g
CaCl2 ·2H2O ...............................................................3.34g
FeSO4 ·7H2O...............................................................99.0mg
(NH4)2MoO4 ................................................................9.25mg
Metals “44”..............................................................50.0mL
Preparation of Hutner’s Mineral Base: Add nitrilotriacetic acid
to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to
6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L.
Metals “44”:
Composition per 100.0mL:
ZnSO4 ·7H2O................................................................1.1g
FeSO4 ·7H2O................................................................0.5g
EDTA.......................................................................0.25g
MnSO4 ·7H2O..............................................................0.154g
CuSO4 ·5H2O...............................................................0.04g
Co(NO3)2 ·6H2O ..........................................................0.025g
Na2B4O7 ·10H2O...........................................................0.018g
Preparation of Metals “44”: Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Pseudomonas species.