Corn Milk Medium

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Corn Milk Medium

培养基配方
Composition per liter:
Skim milk....................................................................20.0g
Agar.........................................................................15.0g
Yeast extract................................................................12.5g
Peptone .....................................................................10.0g
Beef extract..................................................................5.0g
K2HPO4 .......................................................................5.0g
NaCl..........................................................................5.0g
MgSO4 ·7H2O..................................................................1.0g
Corn steep liquor.............................................................7.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat until boiling.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Bacillus subtilis.