Congo Red Agar (CR Agar)

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Congo Red Agar
(CR Agar)

培养基配方
Composition per liter:
GC agar base..............................................................890.0mL
Hemoglobin solution ......................................................100.0mL
Supplement solution........................................................10.0mL
Congo Red (0.01% solution)..................................................0.1mL
pH 7.2 ± 0.2 at 25°C
GC Agar Base:
Composition per 890.0mL:
Agar........................................................................10.0g
Pancreatic digest of casein..................................................7.5g
Peptic digest of animal tissue ..............................................7.5g
NaCl.........................................................................5.0g
K2HPO4 ......................................................................4.0g
Cornstarch...................................................................1.0g
KH2PO4 ......................................................................1.0g
Preparation of GC Agar Base: Add components to distilled/deion-
ized water and bring volume to 890.0mL. Mix thoroughly. Gently heat
until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C.
Hemoglobin Solution:
Composition per 100.0mL:
Hemoglobin...................................................................2.0g
Preparation of Hemoglobin Solution: Add hemoglobin to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Congo Red Solution:
Composition per 100.0mL:
Congo Red...................................................................0.01g
Preparation of Congo Red Solution:  Add Congo Red to
100.0mL of distilled/deionized water. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C.
Supplement Solution:
Composition per liter:
Glucose ...................................................................100.0g
L- Cysteine·HCl............................................................25.9g
L- Glutamine................................................................10.0g
L- Cystine ..................................................................1.1g
Adenine......................................................................1.0g
Nicotinamide adenine dinucleotide ..........................................0.25g
Vitamin B 12 ................................................................0.1g
Thiamine pyrophosphate.......................................................0.1g
Guanine·HCl................................................................0.03g
Fe(NO3)3 ·6H2O.............................................................0.02g
p-Aminobenzoic acid........................................................0.013g
Thiamine·HCl ..............................................................3.0mg
Source: The supplement solution IsoVitaleX ® enrichment is available
from BD Diagnostic Systems. This enrichment may be replaced by
supplement VX from BD Diagnostic Systems.
Preparation of Supplement Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter
sterilize.
Preparation of Medium: To 890.0mL of sterile, cooled GC agar
base aseptically add 100.0mL of sterile, cooled hemoglobin solution,
10.0mL of sterile supplement solution, and 0.1mL of sterile Congo Red
solution. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the isolation and differentiation of virulent and avirulent
strains of Shigella, Vibrio cholerae, Escherichia coli, and Neisseria men-
ingitidis. Used for the detection and differentiation of “iron-responsive”
avirulent mutants. Used in the preparation of live vaccines. Used for the
differentiation of sensitive Neisseria gonorrhoeae (no growth) from
other Neisseria species (growth) that are resistant to Congo Red.