Columbia CNA Agar, Modified with Sheep Blood

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Columbia CNA Agar, Modified with Sheep Blood

培养基配方
Composition per liter:
Columbia blood agar base ................................................950.0mL
Sheep blood, defribinated.................................................50.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Columbia Blood Agar Base:
Composition per liter:
Agar.....................................................................13.5g
Pancreatic digest of casein..............................................12.0g
NaCl......................................................................5.0g
Peptic digest of animal tissue ...........................................5.0g
Beef extract..............................................................3.0g
Yeast extract.............................................................3.0g
Cornstarch................................................................1.0g
Nalidixic acid...........................................................5.0mg
Colistin................................................................10.0mg
Preparation of Columbia Blood Agar Base:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C.
Preparation of Medium:  To 950.0L of cooled, sterile Columbia blood
agar base, aseptically add 50.0mL of sterile, defibrinated sheep blood. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the selective isolation, cultivation, and differentiation of
Gram-positive cocci from clinical and nonclinical materials.