Columbia C.N.A. Agar Base with Blood

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Columbia C.N.A. Agar Base with Blood

培养基配方
Composition per liter:
Biopeptone......................................................................20.0g
Agar ...........................................................................15.0g
NaCl.............................................................................5.0g
Tryptic digest of beef heart.....................................................3.0g
Cornstarch.......................................................................1.0g
Nalidixic acid.................................................................0.015g
Colistin sulfate................................................................0.01g
Sheep blood, defibrinated......................................................50.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium, without blood, is available as a premixed pow-
der from HiMedia.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat until
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 50.0mL of sterile, defibrinated sheep blood to
950.0mL of cooled, sterile agar base. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use: For the selective isolation, cultivation, and differentiation of
Gram-positive cocci from clinical and nonclinical specimens.