Columbia CNA Agar (Columbia Colistin Nalidixic Acid Agar)

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Columbia CNA Agar
(Columbia Colistin Nalidixic Acid Agar)

培养基配方
Composition per liter:
Columbia blood agar base .................................................950.0L
Sheep blood ..............................................................50.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Columbia Blood Agar Base:
Composition per liter:
Agar......................................................................13.5g
Pancreatic digest of casein...............................................12.0g
NaCl.......................................................................5.0g
Peptic digest of animal tissue ............................................5.0g
Beef extract...............................................................3.0g
Yeast extract..............................................................3.0g
Cornstarch.................................................................1.0g
Nalidixic acid...........................................................15.0mg
Colistin.................................................................10.0mg
Preparation of Columbia Blood Agar Base: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C.
Preparation of Medium:  To 950.0mL of cooled, sterile Columbia
blood agar base, aseptically add 50.0mL of sterile, defibrinated sheep
blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the selective isolation, cultivation, and differentiation of
Gram-positive cocci from clinical and nonclinical specimens.