Columbia Blood Agar Base with Rabbit Serum

[所属分类:培养基配方] [发布时间:2020-5-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Columbia Blood Agar Base with Rabbit Serum

培养基配方
Composition per 1020.0mL:
Starch, soluble............................................................9.0g
Resazurin...............................................................10.0μg
Columbia blood agar base ..................................................1.0L
Rabbit serum.............................................................20.0mL
pH 7.3 ± 0.2 at 25°C
Columbia Blood Agar Base:
Composition per liter:
Special peptone...........................................................23.0g
Agar......................................................................10.0g
NaCl.......................................................................5.0g
Starch.....................................................................1.0g
Source: Columbia Blood Agar Base is available as a premixed pow-
der from Oxoid Unipath.
Preparation of Columbia Blood Agar Base: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C.
Preparation of Medium: Combine 1.0L of Columbia blood agar
base with 9.0g of soluble starch and 10.0μg of resazurin. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 20.0mL of sterile
rabbit serum. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the cultivation of Mobiluncus curtisii and Mobiluncus muli-
eris.