CM 3 Broth

[所属分类:培养基配方] [发布时间:2020-5-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CM 3 Broth

培养基配方
Composition per liter:
Cellobiose .....................................................................................6.0g
Sodium citrate...............................................................................3.0g
K2 HPO4 ........................................................................................2.9g
Yeast extract..................................................................................2.0g
KH2PO4 ........................................................................................1.5g
(NH4)2SO4 ....................................................................................1.3g
MgCl2 ·6H2O .................................................................................1.0g
CaCl2 ...........................................................................................0.15g
L -Cysteine·HCl solution...........................................................22.0mL
Resazurin solution......................................................................2.0mL
FeSO4  solution......................................................................... 25.0μL
pH 7.2 ± 0.2 at 25°C
L -Cysteine·HCl Solution:
Composition per 100.0mL:
L -Cysteine·HCl..............................................................................2.5g
Preparation of  L -Cysteine·HCl Solution: Add  L -cysteine·HCl to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C.
Resazurin Solution:
Composition per 10.0mL:
Resazurin ....................................................................................0.01g
Preparation of Resazurin Solution: Add resazurin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly.
FeSO4 Solution:
Composition per 10.0mL:
FeSO4 ............................................................................................0.5g
Preparation of FeSO4 Solution: Add FeSO4 to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Add components, except  L -cysteine·HCl
solution, to distilled/deionized water and bring volume to 978.0mL.
Mix thoroughly. Adjust pH to 7.2. Gently heat and bring to boiling. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Asep-
tically add 22.0mL of sterile  L -cysteine·HCl solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Note: Cellobiose may be replaced by sterile strips of filter paper.
Use:  For the cultivation of Clostridium celerecrescens, Clostridium
papyrosolvens, and Clostridium thermocellum.