Clostridium thermocellum Medium

[所属分类:培养基配方] [发布时间:2020-5-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium thermocellum Medium

培养基配方
Composition per liter:
Cellulose..................................................................10.0g
K2HPO4 ·3H2O ..............................................................2.9g
Cellobiose..................................................................2.0g
Yeast extract...............................................................2.0g
KH2PO4 .....................................................................1.5g
(NH4)2 ·SO4 ...............................................................1.3g
MgCl2 ·6H2O ...............................................................1.0g
CaCl2 .....................................................................0.15g
FeSO4 ·7H2O (5%)........................................................25.0μg
Reductant solution........................................................50.0mL
Resazurin (0.2%) ..........................................................1.0mL
pH 7.8 ± 0.2 at 25°C
Reductant Solution:
Composition per 50.0mL:
NaHCO3 .....................................................................5.0g
L -Cysteine·HCl ...........................................................0.5g
Preparation of Reductant Solution : Add components to dis-
tilled/deionized water and bring volume to 50.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except reductant solu-
tion, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Adjust pH to 7.8. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 50.0mL of sterile reductant solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Clostridium celerecrescens, Clostridium
papyrosolvens, Clostridium stercorarium, and Clostridium thermocel-
lum.