Clostridium perfringens Agar, OPSP (Perfringens Agar, OPSP)

[所属分类:培养基配方] [发布时间:2020-5-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium perfringens Agar, OPSP
(Perfringens Agar, OPSP)

培养基配方
Composition per liter:
Pancreatic digest of casein....................................................15.0g
Agar ..........................................................................10.0g
Liver extract...................................................................7.0g
Papaic digest of soybean meal...................................................5.0g
Yeast extract...................................................................5.0g
Tris(hydroxymethyl)aminomethane buffer..........................................1.5g
Ferric ammonium citrate.........................................................1.0g
Na2S2O5 ........................................................................1.0g
Antibiotic inhibitor .........................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Antibiotic Inhibitor:
Composition per 10.0mL:
Sodium sulfadiazine..............................................................0.1g
Oleandomycin phosphate..........................................................0.5mg
Polymyxin B...................................................................10,000U
Preparation of Antibiotic Inhibitor:  Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except antibiotic inhibi-
tor, to distilled/deionized water and bring volume to 990.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile antibiotic in-
hibitor. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the presumptive identification and enumeration of Clostrid-
ium perfringens in foods.