Clostridium papyrosolvens Medium

[所属分类:培养基配方] [发布时间:2020-5-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium papyrosolvens Medium

培养基配方
Composition per liter:
Paper strips, sterile...................................................3.0g
K2HP4 .................................................................1.65g
NH4Cl ..................................................................1.0g
Yeast extract...........................................................0.6g
L -Cysteine·HCl........................................................0.5g
Resazurin .............................................................1.0mg
Seawater, filtered ..................................................200.0mL
Mineral salt solution ...............................................150.0mL
pH 7.2 ± 0.2 at 25°C
Mineral Salt Solution:
Composition per liter:
(NH4)2SO4 ..............................................................6.0g
NaCl....................................................................6.0g
MgSO4 ·7H2O............................................................1.2g
CaCl2 ·2H2O............................................................0.8g
Preparation of Mineral Salt Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except paper strips, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 7.2 with 5N NaOH. Sparge with 100% N2 . Autoclave for
15 min at 15 psi pressure–121°C. Aseptically and anaerobically add
3.0g of sterile paper strips (filter paper, Kleenex, or lens tissue). Mix
thoroughly. Aseptically and anaerobically distribute into sterile screw-
capped bottles under 100% N2 .
Use: For the cultivation and maintenance of Clostridium papyrosol-
vens.