Clostridium papyrosolvens Medium

[所属分类:培养基配方] [发布时间:2020-5-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium papyrosolvens Medium

培养基配方
Composition per liter:
K2HPO4 .................................................................1.65g
NH4Cl ...................................................................1.0g
Yeast extract............................................................0.6g
L -Cysteine·HCl ........................................................0.5g
Resazurin ..............................................................1.0mg
Seawater, filtered....................................................200.0mL
Mineral salt solution.................................................150.0mL
Cellobiose solution....................................................50.0mL
pH 7.2 ± 0.2 at 25°C
Mineral Salt Solution:
Composition per liter:
(NH4)2SO4 ..............................................................6.0g
NaCl....................................................................6.0g
MgSO4 ·7H2O............................................................1.2g
CaCl2 ·2H2O ...........................................................0.8g
Preparation of Mineral Salt Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Cellobiose Solution:
Composition per 50.0mL:
D- Cellobiose...........................................................5.0g
Preparation of Cellobiose Solution: Add cellobiose to distilled/
deionized water and bring volume to 50.0mL. Mix thoroughly. Sparge
under 100% N2 gas for 3 min. Filter sterilize.
Preparation of Medium: Add components, except cellobiose solu-
tion, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Adjust pH to 7.2 with 5N NaOH. Sparge with 100% N2 .
Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaer-
obically add 50.0mL of sterile cellobiose solution. Mix thoroughly.
Aseptically and anaerobically distribute into sterile screw-capped bot-
tles under 100% N2 .
Use: For the cultivation and maintenance of Clostridium papyrosolvens.