Clostridium lentocellum Agar

[所属分类:培养基配方] [发布时间:2020-5-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium lentocellum Agar

培养基配方
Composition per 1201.0mL:
Agar.......................................................................30.0g
K2HPO4 ....................................................................1.65g
NH4SO4 .....................................................................1.6g
Yeast extract...............................................................1.0g
NaCl.......................................................................0.96g
L -Cysteine·HCl ...........................................................0.5g
CaCl2 ....................................................................96.0mg
MgSO4 ....................................................................96.0mg
Cellulose suspension.....................................................200.0mL
Resazurin (0.1% solution)..................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Cellulose Suspension:
Composition per 100.0mL:
Whatman CF cellulose powder................................................4.0g
Preparation of Cellulose Suspension : Add cellulose powder to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation of Clostridium lentocellum and other
Clostridium species.