Clostridium Cellulolytic Medium

[所属分类:培养基配方] [发布时间:2020-5-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium Cellulolytic Medium

培养基配方
Composition per liter:
Agar.......................................................................20.0g
Cellulose...................................................................7.5g
K2HPO4 ·3H2O ..............................................................2.9g
Yeast extract...............................................................2.0g
KH2PO4 .....................................................................1.5g
(NH4)2SO4 ..................................................................1.3g
FeSO4 .....................................................................1.25g
L -Cysteine·HCl·H2O.......................................................1.0g
MgCl2 ·6H2O ...............................................................1.0g
CaCl2 ·2H2O ..............................................................0.15g
Resazurin..................................................................2.0mg
pH 7.5 ± 0.2 at 25°C
Preparation of Medium:  Add components, except  L -cyste-
ine·HCl·H2O, to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Heat to boiling. Adjust pH to 7.5. Prereduce under
100% N2 . Add  L -cysteine·HCl·H2O. Distribute into tubes under 100%
N2 . Cap tubes with rubber stoppers. Autoclave for 15 min at 15 psi
pressure–121°C.
Use:  For the cultivation and maintenance of Clostridium cellulolyti-
cum and other bacteria that can degrade cellulose.