Clostridium Broth Base

[所属分类:培养基配方] [发布时间:2020-5-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium Broth Base

培养基配方
Composition per liter:
Casein peptone...........................................................15.0g
Meat extract.............................................................10.0g
Yeast extract.............................................................5.0g
Sodium acetate............................................................5.0g
L -Cysteine...............................................................0.5g
Lactate solution........................................................10.0mL
pH 6.0 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Lactate Solution:
Composition per 10.0mL:
Sodium lactate ...........................................................5.0g
Preparation of Lactate Solution:  Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except lactate solution,
to distilled/deionized water and bring volume to 990.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Aseptically add sterile lactate solution, Mix thoroughly. Aseptically
distribute into sterile tubes.
Use: For the identification of spores of Clostridium tyrobutyricum,
which is usually responsible for “late blowing” in cheese.