Brilliant Green Bile Broth with MUG

[所属分类:培养基配方] [发布时间:2020-1-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliant Green Bile Broth with MUG

培养基配方
Composition per liter:
Oxgall, dehydrated......................................20.0g
Lactose.................................................10.0g
Pancreatic digest of gelatin............................10.0g
MUG (4-Methyl umbelliferyl-β- D- glucuronide) .........0.05g
Brilliant Green........................................0.013g
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to1.0L. Mix thoroughly. Distribute into tubes
containing inverted Durham tubes, in 10.0mL amounts for testing
1.0mL or less of sample. Autoclave for 12 min (not longer than 15 min)
at 15 psi pressure–121°C. After sterilization, cool the broth rapidly.
Use: For the detection of coliform microorganisms in foods, dairy
products, water, and wastewater, as well as in other materials of sani-
tary importance. The presence of Escherichia coli and other coliforms
is determined by the presence of fluorescence in the tube.