Brain Heart Infusion, Supplemented (BHIS)

[所属分类:培养基配方] [发布时间:2020-1-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion, Supplemented
(BHIS)

培养基配方
Composition per liter:
Brain heart infusion broth, dehydrated.......................37.0g
Yeast extract.................................................5.0g
Supplement solution ........................................10.2mL
Resazurin (0.25% solution) ..................................4.0mL
pH 7.4 ± 0.2 at 25°C
Supplement Solution:
Composition per 10.2mL:
L -Cysteine·HCl·H2O.........................................0.5g
Hemin solution..............................................10.0mL
Vitamin K 1 solution.........................................0.2mL
Preparation of Supplement Solution:  Add  L -cysteine·HCl·H2O
and vitamin K 1 to 10.0mL of hemin solution. Mix thoroughly.
Hemin Solution:
Composition per 100.0mL:
Hemin........................................................50.0mg
1N NaOH .....................................................1.0mL
Preparation of Hemin Solution:  Add components to 100.0mL of
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin K 1 Solution:
Composition per 30.0mL:
Ethanol (95% solution)........................................30.0mL
Vitamin K 1 ..................................................0.15mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 30.0mL
of 95% ethanol. Mix thoroughly.
Preparation of Medium: Add components (brain heart infusion
broth powder, yeast extract, and resazurin) to distilled/deionized water
and bring volume to 990.0mL. Mix thoroughly and while stirring bring
to boiling for 1 min. Cool to 45°–50°C. Add supplement solution con-
taining hemin solution, vitamin K 1 solution, and  L -cysteine. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes while
agitating gently to distribute the precipitate through the medium.
Use:  For the cultivation of Centipeda periodontii.