Brain Heart Infusion with PABA and Agar, HiVeg

[所属分类:培养基配方] [发布时间:2020-1-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion with PABA and Agar, HiVeg

培养基配方
Composition per liter:
Plant infusion..............................................10.0g
Plant peptone...............................................10.0g
Plant special infusion.......................................7.5g
Na2HPO4 .....................................................2.5g
NaCl.........................................................5.0g
Glucose .....................................................2.0g
Agar ........................................................1.0g
p-Aminobenzoic acid (PABA)..................................0.05g
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. The addition of 1.0g
agar to the medium enhances the growth of anaerobic and microaero-
philic microorganisms. Heat with frequent agitation and boil for 1 min
to dissolve. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For the detection of microorganisms in the blood of patients who
have received sulfonamide therapy.