Brain Heart CC Agar (Brain Heart Cycloheximide Chloramphenicol Agar)

[所属分类:培养基配方] [发布时间:2020-1-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart CC Agar
(Brain Heart Cycloheximide Chloramphenicol Agar)

培养基配方
Composition per liter:
Pancreatic digest of casein................................16.0g
Agar ......................................................13.5g
Brain heart, solids from infusion .........................8.0g
Peptic digest of animal tissue.............................5.0g
NaCl.......................................................5.0g
Na2HPO4 ...................................................2.5g
Glucose ...................................................2.0g
Cycloheximide..............................................0.5g
Chloramphenicol...........................................0.05g
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks while shaking to distribute precipitate. Autoclave for 15 min
at 15 psi pressure–118°C.
Use: For the selective isolation of fastidious pathogenic fungi such as
Histoplasma capsulatum and Blastomyces dermatiditis from speci-
mens heavily contaminated with bacteria and other fungi. It may also
be used as a base supplemented with sheep blood and gentamicin for
enrichment and additional selectivity.